LIU Bo, ZHANG Shujun, YANG Yini, et al. Comparative Analysis on Terpenoids of Extraction Process from Stigma Maydis by UPLC Method[J]. Science and Technology of Food Industry, 2023, 44(14): 282−289. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022080314.
Citation: LIU Bo, ZHANG Shujun, YANG Yini, et al. Comparative Analysis on Terpenoids of Extraction Process from Stigma Maydis by UPLC Method[J]. Science and Technology of Food Industry, 2023, 44(14): 282−289. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022080314.

Comparative Analysis on Terpenoids of Extraction Process from Stigma Maydis by UPLC Method

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  • Received Date: August 30, 2022
  • Available Online: May 21, 2023
  • An extraction process of terpenoids from stigma maydis was discussed in this paper. Firstly, a UPLC analytical method was established based on the three standard substances tigmene F(1), 3β-hydroxy- ent-kaur-15-en-17-oic acid-18-ester(2), and 4α-hydroxy-19-nor-ent-kaur-15-en-17-oic acid (3) from stigma maydis. The chromatography condition was determined as C18 100Ȧ column (4.6 mm×150 mm, 2.6 µm) eluted with MeOH (A)-0.1% phosphoric acid (B) in gradient with the 1.0 mL/min flow rate. The column temperature was 40 ℃ with a 10 μL injection volume. The detection wavelength was set at 220 nm. Results showed that, three terpenoids had good linearity (r>0.9993) in the concentration range of 0.96~192.0 μg/mL (stigmene F) and 5.0~200.0 μg/mL (3β-hydroxy-ent-kaur-15-en-17-oic acid-18-ester and 4α-hydroxy-19-nor-ent-kaur-15-en-17-oic acid), respectively. They showed the same limits of quantitation (LOQ, S/N=10) ranged from 5.0 μg/g to 10.0 μg/g, similar average recovery rate of 89.1%~105.8%, and with could be used for quantitative analysis and provided research reference for terpenoids in stigma maydis. Finally, guided by the optimized analytical method, the extraction process for the three terpenoids from stigma maydis was determined as follows: The extract solvent was 20% MeOH-H2O, which was 25 times weight of stigma maydis, and with refluxed extraction for 1 h.
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  • [1]
    王明华, 石瑞, 赵二劳. 玉米多酚提取工艺及其生物活性研究进展[J]. 中国调味品,2022,47(3):217−220. [WANG M H, SHI R, ZHAO E L. Research progresson extraction technology and bioloactivity of corn polphennols[J]. China Condiment,2022,47(3):217−220.

    WANG M H, SHI R, ZHAO E L. Research progresson extraction technology and bioloactivity of corn polphennols[J]. China Condiment, 2022, 47(3): 217-220.
    [2]
    刘羽婷, 宫春宇, 廉雅雯, 等. 不同预处理方式对玉米须多糖提取及抗氧化活性的影响[J]. 食品科技,2022,47(4):209−215. [LIU Y T, GONG C Y, LIAN Y W, et al. Effects of different pretreatment methods on extraction and antioxidant activity of polysacchardes from corn silk[J]. Food Science and Technology,2022,47(4):209−215.

    LIU Y T, GONG C Y, LIAN Y W, et al. Effects of different pretreatment methods on extraction and antioxidant activity of polysacchardes from corn silk[J]. Food Science and Technology, 2022, 47 (4): 209-215.
    [3]
    JIA Y N, WANG Y J, LI R, et al. The structural characteristic of acidic-hydrolyzed corn silk polysaccharides and its protection on the H2O2-injured intestinal epithelial cells[J]. Food Chemistry,2021,356:129691. doi: 10.1016/j.foodchem.2021.129691
    [4]
    VELAZQUEZ D V O, XAVIERR H S, BATISTA J E M, et al. Zea mays L. extracts modify glomerular function and potassium urinary excretion in conscious rats[J]. Phytomedicine,2005,12(5):363−369. doi: 10.1016/j.phymed.2003.12.010
    [5]
    NAMBA T, XU H, KADOTS, et al. Inhibition of IgE formation in mice by glycoproteins from corn silk[J]. Phytotherapy Research,1993,7(3):227−230. doi: 10.1002/ptr.2650070303
    [6]
    LIU J, WANG C, WANG Z, et al. The antioxidant and free-radical scavenging activities of extract and fractions from corn silk (Zea mays L.) and related flavone glycosides[J]. Food Chemistry,2011,126(1):261−269. doi: 10.1016/j.foodchem.2010.11.014
    [7]
    CHAIITTIANAN R, CHAYOPAS P, RATTANATHONGKOM A, et al. Anti-obesity potential of corn silks: Relationships of phytochemicals and antioxidation, anti-pre-adipocyte proliferation, anti-adipogenesis, and lipolysis induction[J] Journal of Functional Foods, 2016, 23: 497-510.
    [8]
    刘璐, 廖李, 汪兰, 等. 玉米须无糖饮料加工工艺优化及成分分析[J]. 湖北农业科学,2014,53(16):3870−3873. [LIU L, LIAO L, WANG L, et al. Optimizing process of No-sugar corn silk beverage and analyzing its component[J]. Hubie Agricultureal sciences,2014,53(16):3870−3873.

    LIU L, LIAO L, WANG L, et al. Optimizing process of No-sugar corn silk beverage and analyzing its component[J]. Hubie Agricultureal sciences, 2014, 53(16): 3870-3873.
    [9]
    CHOI S Y, LEE Y, KIM S S, et al. Inhibitory effect of corn silk on skin pigmentation[J]. Molecules,2014,19(3):2808−2818. doi: 10.3390/molecules19032808
    [10]
    陈艳军, 高旭珍, 关大勇, 等. 玉米须醇提物对肝纤维化大鼠药效学研究[J]. 中国实验方剂学杂志,2012,18(11):195−199. [CHEN Y J, GAO X Z, et al. Study on the pharmacodynamic of rthanol rxtracts from Lonicera japonica in rats with hepatic fibrosis[J]. Chinese Journal of Experimental Traditional Medical Formulae,2012,18(11):195−199.

    CHEN Y J, GAO X Z, et al. Study on the pharmacodynamic of rthanol rxtracts from Lonicera japonica in rats with hepatic fibrosis[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2012, 18(11): 195-199.
    [11]
    张亚亨, 杨雪军. 中药玉米须的现代药理研究及临床应用进展[J]. 上海中医药大学学报,2022,36(S1):287−290. [ZHANG Y H, YANG X J. Progress in modern pharmacological research and clinical application of traditional Chinese medicine corn silk[J]. Academic Journal of Shanghai University of Traditional Chinese Medicine,2022,36(S1):287−290.

    ZHANG Y H, YANG X J. Progress in modern pharmacological research and clinical application of traditional Chinese medicine corn silk[J]. Academic Journal of Shanghai University of Traditional Chinese Medicine, 2022, 36(S1): 287-290.
    [12]
    滕蓉. 玉米须玫瑰茄复合保健饮料的研制[J]. 山东化工,2021,50(20):36−37,40. [TENG R. Development of compound health drink with corn silk and hibiscus sabdariffa[J]. Shandong Chemical Industry,2021,50(20):36−37,40.

    TENG R. Development of compound health drink with corn silk and hibiscus sabdariffa[J]. Shandong Chemical Industry, 2021, 50(20): 36-37, 40.
    [13]
    江芳, 林菁, 吴晓青. 一种复方藤茶的研制[J]. 食品工业,2021,42(4):47−50. [JIANG F, LIN Q, WU X Q. Preparation of compound ampelopsis grossedentata W. T. Wang Tea[J]. The Food Industry,2021,42(4):47−50.

    JIANG F, LIN Q, WU X Q. Preparation of compound ampelopsis grossedentata W. T. Wang Tea[J]. The Food Industry, 2021, 42(4): 47-50.
    [14]
    黄晓梅, 伍兴武, 吴琼峰, 等. 玉米须多糖的提取及其对面条品质的影响[J]. 粮食与油脂,2021,34(6):64−67. [HUANG X M, WU X W, WU Q F, et al. Extraction of corn silk polysaccharides and effect on noodle quality[J]. Cereals & Oils,2021,34(6):64−67.

    HUANG X M, WU X W, WU Q F, et al. Extraction of corn silk polysaccharides and effect on noodle quality[J]. Cereals & Oils, 2021, 34(6): 64-67.
    [15]
    HUFFAKER A, KAPLAN F, VAUGHAN M M, et al. Novel acidic sesquiterpenoids constitute a dominant class of pathogen-induced phytoalexins in maize[J]. Plant Physiology,2011,156(4):2082−2097. doi: 10.1104/pp.111.179457
    [16]
    李晓雪, 赵明, 马耀玲, 等. 玉米须化学成分研究[J]. 中草药,2021,52(12):3480−3484. [LI X X, ZHAO M, MA Y L, et al. Chemical constituents from style and stigma of Zea mays[J]. Chinese Traditional and Herbal Drugs,2021,52(12):3480−3484.

    LI X X, ZHAO M, MA Y L, et al. Chemical constituents from style and stigma of Zea mays[J]. Chinese Traditional and Herbal Drugs, 2021, 52(12): 3480-3484.
    [17]
    ZHOU W L, LIN B, HOU Z L, et al. Isolation of macrocarpene-type sesquiterpenes from Stigma maydis with neuroprotective activities[J]. Fitoterapia, 2020, 141: 104448.
    [18]
    ZHOU W Y, LIN B, HOU Z L, et al. A new monoterpene-lactone with neuroprotective activity from corn silk[J]. Natural Product Research,2019,10(9):1−4.
    [19]
    徐燕, 邹忠梅, 梁敬钰. 玉米须的化学成分[J]. 中国天然药物,2008(3):237−238. [XU Y, ZOU Z M, LIANG J Y. Chemical constituents from the style Zea mays L

    J]. China Nat Med May,2008(3):237−238.
    [20]
    QI X L, ZHANG Y, ZHAO P, et al. Ent-Kaurane diterpenoids with neuroprotective properties from corn silk (Zea mays)[J]. Journal of Natural Products,2018,81(5):1225−1234. doi: 10.1021/acs.jnatprod.7b01017
    [21]
    SONG X Y, GUO R, QI X L, et al. Terpenoids from stigma maydis (Zea mays L.) alleviate hydrogen peroxide-induced SH-SY5Y cell injury by activating Nrf2[J]. Bioorganic Chemistry,2020,102:104131. doi: 10.1016/j.bioorg.2020.104131
    [22]
    袁兴利, 闫利华, 张启伟, 等. HPLC测定冬凌草中迷迭香酸、冬凌草甲素和猫眼草黄素的含量[J]. 中国中药杂志,2013,38(14):2343−2347. [YUAN X L, YAN L H, ZHANG Q W, et al. Simulaneous determination of rosmarinic acid, oridonin and chryoplenin in Isodon rubescens by HLPC[J]. China Journal of Chinese Materia Medica,2013,38(14):2343−2347.

    YUAN X L, YAN L H, ZHANG Q W, et al. Simulaneous determination of rosmarinic acid, oridonin and chryoplenin in Isodon rubescens by HLPC[J]. China Journal of Chinese Materia Medica, 2013, 38 (14): 2343-2347.
    [23]
    刘安平, 苏媛, 刘学良, 等. HPLC法同时测定山地虎耳草中5种成分[J]. 中国药师,2022,25(10):1826−1829. [LIU A P, SU Y, LIU X L, et al. Simultaneous determination of five constituents in Saxifraga montana by HPLC[J]. China Pharmacist,2022,25(10):1826−1829.

    LIU A P, SU Y, LIU X L, et al. Simultaneous determination of five constituents in Saxifraga montana by HPLC[J]. China Pharmacist, 2022, 25(10): 1826-1829.
    [24]
    王丽荣, 贾文君, 陈明敏, 等. 高效液相色谱法(HPLC)测定芒果多种植物生长调节剂含量[J]. 植物生理学报,2022,58(5):981−988. [WANG L R, JIA W Q, CHEN M M, et al. Determination of contents of several plant growth regulators in mango by high performance liquid chromatography (HPLC)[J]. Plant Physiology Journal,2022,58(5):981−988. doi: 10.13592/j.cnki.ppj.200003

    WANG L R, JIA W Q, CHEN M M, et al. Determination of contents of several plant growth regulators in mango by high performance liquid chromatography (HPLC)[J]. Plant Physiology Journal, 2022, 58(5): 981-988. doi: 10.13592/j.cnki.ppj.200003
    [25]
    毛思浩, 陈碧莲, 李文庭, 等. HPLC测定毛叶香茶菜中迷迭香酸和冬凌草甲素含量[J]. 中国现代应用药学,2016,33(7):922−925. [MAO S H, CHEN B L, LI W T, et al. Determination of rosmarinic acid and oridonin in Rabdosia japonica (Burm. f.)Hara by HPLC[J]. Chinese Journal of Modern Applied Pharmacy,2016,33(7):922−925.

    MAO S H, CHEN B L, LI W T, et al. Determination of rosmarinic acid and oridonin in Rabdosia japonica (Burm. f. )Hara by HPLC[J]. Chinese Journal of Modern Applied Pharmacy, 2016, 33(7): 922-925.
    [26]
    李培照, 李春, 梁婉婷, 等. 高效液相色谱法(HPLC)测定保加利亚乳杆菌胞内Acetyl-CoA[J]. 食品工业科技,2013,34(22):58−61,65. [LI P Z, LI C, LIANG W T, et al. Determination of intracellular acetyl CoA in L. bulgaricus by HPLC[J]. Science and Technology of Food Industry,2013,34(22):58−61,65.

    LI P Z, LI C, LIANG W T. Determination of intracellular acetyl CoA in L. bulgaricus by HPLC[J]. Science and Technology of Food Industry, 2013, 34(22): 58-61, 65.
    [27]
    丁浩然, 刘峰, 陈艳, 等. 急支糖浆一板多药味HPTLC鉴别和HPLC含量测定方法的研究[J]. 中国药房,2022,33(5):555−562. [DING H R, LIU F, CHEN Y, et al. Study on HPLC identification of one-plate multi-drug and HPLC content determination method for jizhi syrup[J]. China Pharmacy,2022,33(5):555−562.

    DING H R, LIU F, CHEN Y, et al. Study on HPLC identification of one-plate multi-drug and HPLC content determination method for jizhi syrup[J] China Pharmacy, 2022, 33 (5): 555-562.
    [28]
    陈两绵, 刘晓谦, 张永欣, 等. 茶藨子叶状层菌中核苷类成分的 UPLC-Q-TOF-MS 定性分析和HPLC含量测定[J]. 中国中药杂志,2021,46(12):2912−2922. [CHEN L M, LIU X Q, ZHANG Y X, et al. Qualitative and quantitatives analysis of nucleobases in phylloporia ribis by UPLC-Q-TQF-MS and HPLC[J]. China Journal of Chinese Materia Medica,2021,46(12):2912−2922.

    CHEN L M, LIU X Q, ZHANG Y X, et al. Qualitative and quantitatives analysis of nucleobases in phylloporia ribis by UPLC-Q-TQF-MS and HPLC[J]. China Journal of Chinese Materia Medica, 2021, 46 (12): 2912-2922.
    [29]
    海丽娜, 王鹏飞, 王京辉, 等. 复方苦参注射液UPLC指纹图谱检测及6种成分含量测定[J]. 中国药学杂志,2022,57(14):1198−1204. [HAI L N, WANG P F, WANG J H, et al. Study on the UPLC fingerprint and determination of six components of compound Kushen injection[J]. Chinese Pharmaceutical Journal,2022,57(14):1198−1204.

    HAI L N, WANG P F, WANG J H, et al. Study on the UPLC fingerprint and determination of six components of compound Kushen injection[J]. Chinese Pharmaceutical Journal, 2022, 57 (14): 1198-1204.
    [30]
    何民友, 段志文, 杨洁, 等. 基于UPLC指纹图谱及2种化学成分含量测定的月季花质量分析[J]. 天然产物研究与开发,2022,34(9):1455−1464. [[HE M Y, DUAN Z W, YANG J, et al. Quality analysis of Rose Chinesis flos based on UPLC fingerprint and content determination of two chemical components[J]. Natural Product Research and Development,2022,34(9):1455−1464.

    [HE M Y, DUAN Z W, YANG J, et al. Quality analysis of Rose Chinesis flos based on UPLC fingerprint and content determination of two chemical components[J]. Natural Product Research and Development, 2022, 34 (9): 1455-1464.

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