Preparation of Zn2+ Complex of Total Flavonoids from Fruit of Rosa davurica Pall and Its Antioxidant Activity in Vitro
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1. School of Chemistry and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China;
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2. College of Chemistry, Jilin University, Changchun 130000, China
More Information
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- Received Date:
December 11, 2018
- Available Online:
November 12, 2020
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Abstract
The preparation process of Zn2+ complex of total flavonoids from fruit of Rosa davurica Pall.(FRDP)and its antioxidant activity in vitro were explored in this work. Taking the conversion rate of total flavonoids as index,the preparation procedures of Zn2+ complex of total flavonoids from FRDP were investigated by using single factor combined with orthogonal experiments. The scavenging ability for ·OH,O2-·,anti-liposome peroxidation capability and ABTS+· of total flavonoids and its Zn2+ complexes were tested to evaluate the antioxidant activity in vitro. Results showed the optimal preparation process of Zn2+ complex of total flavonoids were as follows:14 mg/mL of zinc chloride solution,8 mg/mL of total flavonoids solution,the pH was adjusted to 9,followed by the shaking at 50℃ for 3.0 h,the resulting mixture was centrifuged,and total flavonoids Zn2+ complex of FRDP was obtained by collecting the precipitation,in which the conversion rate reached 96.94% with a yield of 35.76%.Compared with total flavonoids of FRDP and VC solution,Zn2+ complex had superior scavenging ability for ·OH,O2-· and excellent anti-liposome peroxidation capability,and it also had good ABTS+· clearing ability. Therefore,Zn2+ complex of total flavonoids from FRDP prepared in this work showed a certain antioxidant capacity.
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References
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