HU Xiang- dong, PAN Ling- yan, YE Mao, HU Wei-qing. Screening and fermentation optimization of a high-yield-astaxanthin-producing Phaffia rhodozyma[J]. Science and Technology of Food Industry, 2016, (05): 142-147. DOI: 10.13386/j.issn1002-0306.2016.05.019
Citation: HU Xiang- dong, PAN Ling- yan, YE Mao, HU Wei-qing. Screening and fermentation optimization of a high-yield-astaxanthin-producing Phaffia rhodozyma[J]. Science and Technology of Food Industry, 2016, (05): 142-147. DOI: 10.13386/j.issn1002-0306.2016.05.019

Screening and fermentation optimization of a high-yield-astaxanthin-producing Phaffia rhodozyma

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  • Received Date: June 04, 2015
  • Objective: To screen out a high- yield- astaxanthin- producing Phaffia rhodozyma and optimize the culture medium conditions.Method: Phaffia rhodozyma strain( P.rhodozyma 02) was used as original strain treated with UV- Li Cl and60Co- γ mutagenesis respectively.Mutant strains with higher astaxanthin productivity were screened out and used as the starting strains for genome shuffling. After 5 rounds of protoplast fusion,obtained the best recombinant with further high yield of astaxanthin.The optimum culture medium conditions was obtained by adding natural promoter rich in synthetic precursor of astaxanthin to the medium and optimizing the parameter of culture medium.Result: a high- yield- astaxanthin- producing recombinant F5- 9 was obtained and the optimum culture medium conditions were: glucose 30.0 g / L,peptone 3.0 g / L,yeast extract powder 7.0 g / L,( NH4)_2SO45.0 g / L,Mg SO4·7H2O 2.75 g / L,KH2PO4 1.5 g / L,Ca Cl2·2H2O 0.2 g / L,carrot juice 150 mg / L. Eventually the astaxanthin production reached at( 83.39 ± 1.03) mg / L,which was 17.56 times than the original strain. Conclusion: The traditional mutation and genome shuffling can significantly improve the yield of astaxanthin and there is room for further improvement in the yield of astaxanthin by adding natural promoter rich in synthetic precursor of astaxanthin carrot juice into culture medium.
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  • [1]
    Hu C,Han W,Chen Z,et al.Statistical optimization of microwave-assisted astaxanthin extraction from Phaffia rhodozym[C].Yan Tai:2010 3rdInternational Conference on BiomedicalEngineering and Informatics,2010,5:2104-2109.
    [2]
    Mezquita P C,Huerta B B,Ramírez J P,et al.Milks pigmentation with astaxanthin and determination of colour stability during short period cold storage[J].Journal of Food Science and Technology,2015,52(3):1634-1641.
    [3]
    汪洪涛,徐学明,金征宇.天然虾青素对金鱼体色变化的影响[J].现代食品科技,2013,29(4):749-751.
    [4]
    Tominaga K,Hongo N,Karato M,et al.Cosmetic benefits of astaxanthin on humans subjects[J].Acta Biochimica Polonica,2012,59(1):43-47.
    [5]
    Yoshida H,Yanai H,Ito K,et al.Administration of natural astaxanthin increases serum HDL-cholesterol and adiponectin in subjects with mild hyperlipidemia[J].Atherosclerosis,2010,209(2):520-523.
    [6]
    胡向东,潘玲燕,章祺,等.复合诱变选育高产虾青素的红法夫酵母菌株[J].食品与发酵工业,2014,40(10):58-68.
    [7]
    龚玉姣,屈平华,曹理想,等.协同诱变法选育虾青素高产优良酵母菌株[J].微生物学杂志,2010,30(1):16-20.
    [8]
    Stemmer W P C.Rapid evolution of a protein in vitro by DNA shuffling[J].Nature,1944,370(6488):389-391.
    [9]
    Zhang Y X,Perry K,Vinci V A,et al.Genome shuffling leads to phenotypic improvement in bacteria[J].Nature,2002,415(6872):644-646.
    [10]
    Wang Y H,Li Y,Pei X L,et al.Genome shuffling improved acid tolerance and L-lactic acid volumetric productivity in Lactobacillus rhamnosus[J].Journal of Biotechnologica,2007,129(3):510-515.
    [11]
    Wang M Z,Liu S S,Li Y Y,et al.Protoplast mutation and genome shuffling induce the endophytic fungus Tubercularia sp.TF5 to produce new compounds[J].Current Microbiology,2010,61(4):254-260.
    [12]
    胡向东,朱静,梁新乐.一株高产天然虾青素的红法夫酵母菌株及其选育方法和应用[P].中国专利,201210321949.2.2013-01-09.
    [13]
    郑宗宝.基于基因组重排技术的红法夫酵母虾青素高产株选育[D].天津:天津大学,2009.
    [14]
    曲秋皓,宁国赞.红色酵母原生质体形成和再生条件的研究[J].工业微生物,1998,28(4):38-41.
    [15]
    张丽敏,华艳艳,孙玉梅,等.红发夫酵母生物合成虾青素天然促进剂的研究[J].河南工业大学学报2011,32(1):66-69.
    [16]
    朱晓立,梁世中,邓毛程.几种物质对红发夫酵母生长和虾青素合成的影响[J].食品科技,2008,33(12):20-23.
    [17]
    魏希颖,王家稳,刘清梅,等.基因组重排技术在微生物菌种选育中的应用[J].陕西师范大学学报,2013,41(5):68-72.

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