Antioxidant Activity of Total Flavonoids from Tartary Buckwheat Bran in Vitro and Its Effect on Antialcoholism and Liver Protection in Vivo

Objective:To study the antioxidant activity in vitro,and relieving alcoholism and hepatoprotective effect in mice by total flavonoids from tartary buckwheat bran(TFTB). Method:In vitro antioxidant experiments,the scavenging effects of TFTB on superoxide anion radical(O₂^-·),DPPH·and NO₂^- were observed. In the alcohol-relieving and hepatoprotective experiments,fifty mice were randomly divided into drunkenness model group,positive control group(150 mg/kg·bw),TBTF low,medium and high dose group(100,200 and 300 mg/kg·bw). After a one-time gavage of vehicle or drug for 30 min,the mice in each group were given a one-time gavage of Luzhou-flavor liquor of 38%vol(0.20 mL/10 g),and then the drunkenness rate,tolerance time of drunkenness,time of drunkenness and the levels of aspartate aminotransferase(AST)in serum,malondialdehyde(MDA)and superoxide dismutase(SOD)in liver were determined. Results:TFTB had a certain scavenging effect on superoxide anion radical(O₂^-·),DPPH· and NO₂^-,and the inhibitory concentration 50%(IC₅₀)values were 1.36,0.02 and 1.79 mg/mL. Compared with the model group of drunkenness,the tolerance time of drunkenness in the high dose group of TFTB was significantly prolonged(P<0.05),the groups of TFTB also had a shortened drunkenness(P<0.01),a significant decrease of AST level in serum(P<0.05),and a significant increase SOD activity(P<0.01)and a significant decrease of MDA level in liver(P<0.05). Conclusion:TFTB shows strong antioxidant activity in vitro,and has a certain anti-alcoholic effect and can alleviate alcoholic liver injury in mice to a certain extent.