WANG Yu, YANG Hao-bo, ZHONG Shuai, LIU Xin, SHENG Yu-jun, LI Tong, ZONG Cheng-wen. Determination of soluble sugar in Vaccinium uliginosum fruit in Changbai Mountain by HPLC-ELSD[J]. Science and Technology of Food Industry, 2020, 41(2): 238-243. DOI: 10.13386/j.issn1002-0306.2020.02.038
Citation: WANG Yu, YANG Hao-bo, ZHONG Shuai, LIU Xin, SHENG Yu-jun, LI Tong, ZONG Cheng-wen. Determination of soluble sugar in Vaccinium uliginosum fruit in Changbai Mountain by HPLC-ELSD[J]. Science and Technology of Food Industry, 2020, 41(2): 238-243. DOI: 10.13386/j.issn1002-0306.2020.02.038

Determination of soluble sugar in Vaccinium uliginosum fruit in Changbai Mountain by HPLC-ELSD

  • Objective:To analyze the edible value of Vaccinium uliginosum in Changbai Mountain and provide theoretical basis for production,processing and germplasm conservation and utilization. Methods:The soluble sugar contents in 40 V. uliginosum fruits from two different populations was determined and compared by high performance liquid chromatography-evaporative light scattering detector(HPLC-ELSD). The conditions of high performance liquid chromatography were CAPCELL PAK NH2 UG80 S5(4.6 mm×250 mm,5 μm)column,acetonitrile-water as mobile phase(75:25,v:v),flow rate 1.0 mL·min-1,column temperature 35℃ with evaporative light scattering detector(ELSD),drift tube temperature 80℃. Carrier was high purity N2,N2 flow rate was 1.0 L·min-1,gain value was 1,injection volume was 5 μL. Results:The soluble sugar was well separated under the selected chromatographic conditions. The correlation coefficients of the standard curves were over 0.9994,and the recoveries ranged from 102.83% to 106.29%. Fructose and glucose were the main sugars in V. uliginosum fruit of Changbai Mountain,and fructose content was the highest. The total sugar and sweetness values of Dongfanghong Forest Farm at high altitude were significantly(P<0.05)higher than those of Lanjia Forest Farm at low altitude,and DFH-11 was the sample with the highest total sugar and sweetness values. Conclusion:The method had the characteristics of fast operation and good repeatability,and could accurately determine the types and contents of sugar in V. uliginosum fruit. The V. uliginosum fruit can be processed and utilized as the preferred material for breeding.
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