Design of Mobile Packed-Bed Solid-State Fermentation Reactor and Pilot-Scale Fermentation of Tannase

Based on the analysis of tannase solid-state fermentation technology and combination with the structure of traditional thick-layer ventilation starter,a mobile packed-bed solid-state reactor was designed. Using tea stalk as substrate,the pilot-scale fermentation of tannase was carried out in the packed-bed solid-state reactor,and the effects of different ventilation rates of 10,15,20 m³/h on the fermentation were investigated. The results showed that under the conditions of ventilation of 10,15 and 20 m³/h,the maximum enzyme activities reached 8.21,8.08,8.41 U·gds^-1,respectively,and the time of maximum enzyme activities were 96,120,144 h,respectively. Production of tannase by fermentation with tea stalk as substrate in the packed-bed solid-state fermentation reactor,not only had a wide range of raw materials and low price,but also could effectively improve the tannase activity.