Inhibition Effect of Terminalia chebula Retz on Oxidative Modification of apoB100 in LDL

In order to study the inhibition effect of Terminalia chebula Retz(TCR)on oxidative modification of the protein apoB100 contained in low-density lipoprotein(LDL),this paper studied the inhibition effect of the crude extract of TCR(CET)and the effective anti-oxidation part of TCR(EAFT)on the oxidative modification of the protein apoB100 contained in LDL by using qualitative and quantitative fluorescence techniques. The results showed that,CET could significantly(P<0.05)inhibit the decreasing of free amino activity of Lysine in apoB100,which showed a corresponding dose-effect relationship. The reactivity decreasing of free aminosin Lysine of apoB100 was remarkably inhibited by EAFT within the concentrations of 1.25~5.00 μg/mL(P<0.05). Trp fluorescence quenching was markedly inhibited by EAFT within the concentrations of 5~15 μg/mL(P<0.05). The generation of the total fluorescence products and lipofuscins were markedly inhibited by EAFT with the same concentrations. The inhibition effect of EAFT was intuitively showed on the three-dimensional fluorescence contour spectroscopy during LDL oxidation. This study would lay the foundation for further research and development of functional foods with antioxidant of LDL and anti-atherosclerosis.