Evaluation of Antioxidant and Anti-aging Effect of Pueraria Water Extracts and Pueraria Fermentation Broth in Vitro

In this research,pueraria was used as the research object and extracted by water in the means of microbe extraction methods,then the pueraria water extracts(PWE)and pueraria fermentation broth(PFB)were obtained. The two samples were tested for bioactive components,chemical and cellular antioxidant properties including scavenging capacity against 2,2-diphenyl-1-picrylhydrazyl(DPPH)free radical and catalase activity(CAT),and their effects on glutathione peroxidase activity,matrix metalloproteinase-1(MMP-1)and type I collagen(COLI)contents in the culture supernatants of human skin fibroblast cells were examined. The flavonoid content of PWE was(3.47±0.23) mg/mL,and the flavonoid content of PFB was(4.80±0.32) mg/mL.Both of them had obvious DPPH free radical scavenging ability. The IC₅₀ of DPPH free radical scavenging activity of PWE was 2.98 mg/mL. The IC₅₀ of DPPH free radical scavenging acticity was 3.51 mg/mL. Furthermore,the effect of scavenging DPPH was more significant at the same concentration of 2.67~4.44 mg/mL.(p<0.05). The two samples in the concentration range of 0.05~5.00 mg/mL showed no strong cytotoxicity to human skin fibroblast cells and the cell viability was above 85% in the presence of each sample. Stimulation with the samples significantly(p<0.05)increased catalase activity and glutathione peroxidase activity,and the PWE showed significantly(p<0.05) acticity than PFB. Furthermore,both samples could reduced MMP-1 contents,and increased COL I contents after the stimulation with the samples. PFB showed stronger acticity of reducing MMP-1 and increasing COL I(p<0.05). Therefore,PFB and PWE had potential anti-aging effect.