Optimization of Synthesis Technology of Selenium Polysaccharide Ester from Pomegranate Seeds and Its Anti-lipid Oxidation Ability

Polysaccharide of pomegranate seeds and sodium sulfite as raw materials,contents of Se in synthetic product and yields of synthetic product as investigation index,raw material ratio,reaction temperature,concentration of HNO₃ and reaction time as experimental factors,the synthesis conditions were optimized by single factor test and Box-Behnken experimental designs,and simultaneously the product structure and antioxidant capacity were studied. The results showed that,the optimum preparation conditions of selenium polysaccharide ester of pomegranate seeds were as follows:Raw material ratio was 1:1.02,reaction temperature was 76 ℃,concentration of HNO₃ was 0.74%,and reaction time was 9.3 h. Under this conditions,contents of Se in synthetic product was(4.117±0.02) mg/g,and yields of synthetic product was 42.596%±0.13%. UV,IR and TGA analysis fully confirmed that selenium polysaccharide ester of pomegranate seeds contained Se=O,Se-O and Se-C. Synthetic product had a certain antioxidant capacity,so it could significantly slow the trend of oil POV value increasing trend,and had a certain amount of concentration-dependent manner. The study provided a theoretical basis for the full development and utilization of pomegranate seeds resources.