Optimization of Extraction Process of Chlorophyll from Kiwifruit by Response Surface Method

Taking Yichang green kiwifruit pulp as experimental material,using organic solvent extraction,basing on single factor experiments with OD₆₆₆ as an indicator,according to the Box-Behnken design,a 4-factor 3-level response surface analysis method was used to optimize chlorophyll extraction conditions,and the significance and interaction of various factors(concentration of extraction solvent,liquid to material ratio,extraction time,extraction temperature)were analyzed. Results showed that extraction time and extraction temperature were extremly significant factors affecting chlorophyll extraction(p<0.01).The optimized process parameters were as follows:extraction solvent was ethyl acetate,liquid/solid ratio was 1.22:1 mL/g,ethyl acetate volume fraction was 59.6%,extraction time was 236 min,extraction temperature was 59 ℃,absorbance value was 0.636±0.01. At this time,the chlorophyll content was 27.73 μg/g as measured by the Arnon method.