Optimization of Simultaneous Extraction for Rubusoside and Polyphenols from Sweet Tea

Distilled water was used as extraction solvent, response surface methodology was used to optimize the simultaneous extraction process of rubusoside and polyphenols from dried leaves of Guangxi sweet tea. According to the Box-Behnken design method, the extraction rate of rubusoside and sweet tea polyphenols were taken as the response values respectively, the best process of simultaneous extracting rubusoside and sweet tea polyphenols was:The extraction temperature was 57℃, the ratio of liquid to material was 25:1 mL/g, the extraction time was 36 min, and the extraction rate of sweet tea polyphenols was 85.32% (RSD=1.42%, n=3), the extraction rate of rubusoside was 83.58% (RSD=2.24%, n=3). The results provided an experimental basis for the comprehensive utilization of sweet tea.