Effect of Temperature Treatment on the Expression of cspc mRNA and Growth Rate of Lactobacillus rhamnosus

Through the method of temperature treatment on Lactobacillus rhamnosus (LGG) fermentation process, the expression of cold shock protein C (cspc) mRNA had been investigated, and the change of growth rate was investigated. With different temperature and time conditions, cspc mRNA expression was detected. The LGG was induced at the 4th and 8th hours with temperature in fermentation culture, growth rateswere detected, and the freeze-drying survival rate was tested. The results showed that the cspc mRNA expression was significantly decreased by temperature higher than 37℃ induction, and the expression was lowest after induction at 43℃ for 30 min, the growth rate was significantly increased after induction, the freeze-dried survival rate was not decreased. In summary, temperature higher than 37℃ induction could decrease the expression of cspc mRNA and increase the growth rate of LGG.