Optimization of Extraction Process of Active Components from Red Raspberry Seeds

To evaluate the influence of ethanol concentration,extraction time,extraction temperature,material/solvent ratio and pH on active components extraction,tests of single factors and response surface were designed to optimize extraction process of active substances in red raspberry seeds using fat-free Autumn Bliss raspberry seeds as raw materials. The results showed that the optimum extracting conditions were as follows,extraction time of 3.5 h,the ratio of material to solvent 1:50 (g/mL),extraction temperature of 51℃,ethanol concentration of 57% and pH=3. Under these conditions,the extraction concentration of polyphenol extracted from red raspberry seeds was up to(38.32±0.25) mg/g,the flavonoids extraction was(17.50±0.14) mg/g,and the proanthocyanidins extraction was(19.71±0.27) mg/g. The process is stable and reliable. It can provide a theoretical basis for the comprehensive utilization of red raspberry seeds.