Optimization on Aqueous Enzymatic Extraction Process of Protein from the Seeds of Carica papaya L. by Response Surface Methodology

Effects of pH,temperature,time and enzyme amount on the extraction rate of protein from the seeds of Carica papaya L. were established by single-factor tests. Protein was extracted by aqueous enzymatic method and Box-Benhnken design and response surface method was used to optimize the extraction process on the basis of single-factor tests. According to this model,the optimal parameters of extracting protein from papaya seeds were determined as solvent/material ratio 1:19,extraction temperature 49℃,hydrolysis time 5 h,enzyme amount 2%,pH7.0 with papain. The extraction rate under this optimum condition was 84.96%,which would produce new method for the development and utilization of papaya.