Inactivation effect and dynamic model of inactivation kinetics of Vibrio parahaemolyticus by pulsed light

Pulsed light inactivation models of Vibrio parahaemolyticus were studied in order to describe and predict the inactive effects.In order to explore the inactivation effect of pulsed light on Vibrio parahaemolyticus in 3.0% sodium chloride system,the number of Vibrio parahaemolyticus were measured after pulsed light at various flash fluence leves(67,92,113 mJ/cm²) for 0~25 s,respectively.Linear,Weibull and Log-logistic models were used to describe the pulsed light inactivation model.Four criteria,accuracy fact (A_f), bias factor(B_f), root mean square error (RMSE) and regression coefficients (R²) were used to compare the Linear,Weibull and Log-Logistict models,respectively.The results showed that when the irradiation distance was 30 cm and the fluence was 113 mJ/cm² and treatment time was 25 s the Vibrio parahaemolyticus was reduced by 5.63 log CFU/mL, which the sterilization rate was 99.96%.Compared with Linear model,Weibull model and Log-logistic model could better fit the bactericidal curve of Vibrio parahaemolyticus treated by pulsed light.The Weibull model can best fit the dynamics process of pulsed light sterilization,which is simpler,more flexible and practical than the Log-logistic model.