Determination of organic acids in the fermentation process of cabbage kimchi by HPLC

A high performance liquid chromatographic(HPLC)method was established for the simultaneous determination of 9 organic acids in kimchi. This method was then used to determine the contents of organic acids in the fermentation process of cabbage kimchi. The procedure of HPLC analysis was carried out on a Agilent TC-C₁₈ column using methanol and 0.01 mol/L potassium dihydrogen phosphate(3∶97)(pH2.80)as the mobile phase. The flow rate was 1 mL/min and the detection wavelength was 210 nm. The nine organic acids were effective separated and determined under the chosen experimental conditions. Relative standard deviations(RSD)of precision was 0.06%~0.26%(n=6)and RSD of repeatability was 0.67%~4.85%(n=6).The recovery rates were in the range of 95.23%~104.76%. The method was convenient,rapid,accurate and applicable to determine organic acids in kimchi.