Response surface methodology for optimization of ultrafiltration condition for purification of sika deer antler base peptide

The peptide was separated through ultrafiltration from sika deer antler base. The major factors such as pressure, operation time, and material-liquid ratio were investigated to evaluate the effect on infiltration flux.Based on one-factor-at-atime method, Box-Behnken response surface methodology ( RSM) was taken to optimize the key factors that affect the effect of the sika deer antler peptide ultrafiltration. The optimal separation condition was 1.44% of material-liquid ratio, 0.13 MPa of pressure, and 60 min of operation time.Under this condition, the infiltration flux could be reached 1.72 L/m2·h, peptide content in separated samples was 45.2%, better purification effect was achieved.