Study on effect of debittering shaddock peel by ultrasound-assisted alkaline solution-enzymolysis method

The ultrasound-assisted alkaline solution-enzymolysis method, which used removal rate of naringin as the index, was studied to get better debittering effect in the further-processing product of shaddock peel. By single factor experiment and orthogonal experiment, the results showed that the optimum treatment conditions of ultrasound-assisted alkaline solution were obtained as follows: material-to-liquid ratio 1 ∶ 30 ( g/m L) , ultrasonic treatment time 20 min, ultrasonic temperature 55 ℃, ultrasonic power 120 W.Under these optimum conditions, the removal rate of naringin was 75.31%.On this basis, by response surface design, the results showed that 90.14% removal rate of naringin was achieved through the use of naringinase at 0.15%, dosage for 36 min, p H4.1 and 52 ℃. The results of this study indicated that the ultrasound-assisted alkaline solution-enzymolysis method was more effective compared with the traditional naringinase enzymolysis method.