Optimization the extraction process of polysaccharide by response surface methodology from the Tremella fuciformis pedicel and its antioxidant activity

Optimization of hot water extraction of polysaccharide from Tremella fuciformis pedicel( TPP) were investigated using the single- factor test and the response surface methodology( RSM) in the paper.The property structure and antioxidant activities of TPP were analyzed after the crude polysaccharide was preliminarily separated by Sevage method.The results showed that the optimum technological conditions were extraction temperature 92 ℃,extraction time 4.2 h,liquid- solid ratio of 41∶ 1( m L / g),and the yield of TPP achieved the maximum of( 23.41 ± 0.92) mg / g.The evaluation of antioxidant activity suggested that TPP exhibited good antioxidant activity,and there was a certain dose- effect relation between polysaccharide concentration and eliminating rate.The EC50 for reducing power and scavenging activities against ABTS+and DPPH radical were 4.94,4.04,and6.59 mg / m L,respectively.