An assessment on the individual & collective equol cultivating productivity of intestinal bacteria

Five common probiotics including Lacticacid bacteria,Lacticacid bacillus,Bifidobacterium,Bacillus subtilis,Streptococcus thermophilus and LJ-G_1 and LJ-Q_2 isolated from vegetarian feces were used as test bacteria.Isoflavone was added in the medium,the quantitative and qualitative analysis of the equol in medium was investigated by ultraviolet spectroscopy,which would be the basis of the ability to equlol transformability. Then the synergistic effects of the bacteria that can produce equol were inspected to a scale,and probe into the best culture condition of the compound bacteria. The results showed that Lacticacid bacteria,Streptococcus thermophilus,LJ-G_1,LJ-Q_2 can produced equol separately,and bacteria LJ-G_1,LJ-Q_2 from vegetarian have a high capacity to produce equol with 15.92 μg / m L and 14.43 μg/mL. Lacticacid bacteria,they also had synergistic effects when they were recombined which produced equol between 19.04~20.78 μg/mL.The best composite ratio was1∶1,the p H of the medium was 7.0,culture temperature was 37 ℃,can produce equol was 21.23 μg/mg.The enteric bacteria from vegetarian feces can produce equol separately highly.