Optimization of double enzymatic nobiletin extraction by response surface methodology

Citrus skin as raw materials,establishs the method UV spectrophotometric to study the best technique that nobiletin extracted by response surface double enzymatic. By UV spectrophotometry content of citrus peel Nobiletin,and on the basis of one factor test,explore cellulase and pectinase complex ratio of enzyme dosage,Water bath temperature,reaction time,concentration of ethanol,liquid ratio on nobiletin yield,response surface analysis and design optimization methods. The results show that the impact nobiletin yield strength of the order:ethanol concentration > water bath temperature > enzymolysis time > the material- liquid ratio. After the response surface optimization optimal conditions for cellulase and pectinase complex ratio of 1.5 ∶ 1,the amount of enzyme added 5%,the ethanol concentration was 70%,Water bath temperature was 61 ℃,the enzymolysis temperature time was 2 h,the material- liquid ratio was 1 ∶ 15,resulting nobiletin yield 265.1 μg / g. Compared with the predicted value of response surface,the proof test gained the free oil yield was 2.3%,which indicates that the extraction parameters optimized to give accurate and reliable,was suitable for citrus peel extract nobiletin fast and efficient way.