Extraction of total flavonoids from Chmonathus salicifolius S.Y.H leaves by complex enzymatic hydrolysis assisted iltrasonic and its antioxidant activity

The extraction of total flavonoids from Chmonathus salicifolius S.Y.H leaves by complex enzymatic hydrolysis assisted ultrasonic and its antioxidant activity were studied,the optimum enzyme on the extraction amount of total flavonoids from Chmonathus salicifolius S.Y.H leaves were cellulose and pectinase,and the ratio of cellulose and pectinase was 3∶ 1.The optimum extraction conditions of total flavonoids from Chmonathus salicifolius S.Y.H leaves were determined by response surface methodology.It was concluded that ultrasonic extraction power for 290 W,ultrasonic extraction time for 24 min,ethanol concentration for 90%,and the solution and material ratio for27( m L / g). And the extraction amount of total flavonoids from Chmonathus salicifolius S.Y.H leaves reached83.1 mg / g. The flavonoids from Chmonathus salicifolius S.Y.H leaves had good antioxidant activity in vitro,the oxidation experiments showed that the IC50 of DPPH· and ·OH were 1.22 mg / m L and 0.92 mg / m L,so the flavonoids from Chmonathus salicifolius S.Y.H had good antioxidant activity.