Synthesis of cinnamyl acetate by lipase- catalyzed trans- esterification

One novel gene encoding a lipase L- 1 was cloned from the genome of Streptomyces sp.SCSIO 13580 identified from the deep sea of the South China Sea.L- 1 contains 1005 bp and encodes a putative lipase that harbors 334 amino acids and exhibits the highest similarity of 51% with other lipases.The lipase was heterologously expressed in E.coli.The lipase could catalyze the trans- esterification reaction of vinyl acetate and cinnamyl alcohol in non- aqueous medium.The effects including cinnamyl alcohol / vinyl acetate ratio( mol / mol),temperature,solvents and aclyl donors,on the catalytic activities were further investigated.The optimum enzymatic working conditions were obtained as follows: vinyl acetate as the acyl donor,n- hexane as the solvent,ratio of cinnamyl alcohol / vinyl acetate 1 ∶ 6,reaction temperature of 40 ℃,the maximum conversion rate could reach 48.3%,the activity of enzyme powder was 5.59 U / g.