Antioxidant activities in vitro of pine nut coated-film extracts from Pinus koraiensis

It was to find out the antioxidant activities of Pinus koraiensis pine nut coated-film from Changbai Mountain. 60% ethanol and 50% acetone were used,and the extracts were obtained by vacuum freeze-drying.The purity of total polyphenol and total flavonoids of the extracts were measured by spectrophotometry. The free radical scavenging abilities and reducing iron power also were studied in this experiment. The results showed that polyphenol purity of pine nut coated-film ethanol extract was 39.02%,the flavonoid purity was10.19%. The polyphenol purity of acetone extract was 44.09%,the flavonoid purity was 14.59%. The scavenging capacity of pine nut coated-film extract on ABTS⁺·,DPPH· and hydroxyl radical(·OH) was of dose-dependent relationship including the ability of reducing iron power. Its scavenging effects on DPPH· and the ability of reducing iron power were better than that of BHT. And scavenging effects of acetone extracts on DPPH· and·OH were notable better than that of ethanol extracts. These results showed that the antioxidant capacity of acetone extracts was higher than that of ethanol extracts. At the same time,there was positive correlation between the antioxidant capacity of Korean pine nut coated-film extracts and the purity of total polyphenol and total flavonoids in experiment extent.