Optimization of enzymatic hydrolysis conditions for extracting sulforaphane from waste of carmine radish

In this study,sulforaphane was extracted from the waste of carmine radish.In order to enhance the yield of sulforaphane,enzymatic hydrolysis conditions of glucosinolates in waste of carmine radish were optimized.Based on single- factor experiments,the effects of four factors( the hydrolysis temperature,hydrolysis time,amount of VC,p H value) on the yield of sulforaphane were investigated by response surface methodology.Experimental results showed that,when the hydrolysis temperature was 31 ℃,hydrolysis time was 7.7 h,amount of VCwas0.24 mg / kg,p H was 5.6,the yield of sulforaphane was the highest with the value of 0.05613%,which were according with the predicted value of model.This indicated that extraction conditions which analyzed by response surface method were more reliable.