Research of the optimization in parameters of casein production with milk- clotting enzyme from Mucor miehei

Fresh milk was selected as the raw material and Mucor miehei chymosin was used as the coagulant to prepare rennet casein.First,the effects of addition volume of enzyme and Ca Cl2,p H and temperature on the yield of rennet casein were studied using the single factor experiment. Subsequently,the technological parameters of casein production with milk- clotting enzyme from Mucor miehei were further optimized by Box- Behnken design.The results showed the optimum technological parameters were chymosin addition amount of 0.49%,Ca Cl2 addition amount of 0.37 mg / m L,p H6.08 and curd temperature of 35 ℃.Under the optimal conditions,the maximum yield of rennet casein was 3.39%. The study could provide reference basis for the full utilization of Mucor miehei chymosin in the production of rennet casein.