Study on mutation breeding and identification of lactic acid bacteria for γ- aminobutyric acid production
-
Graphical Abstract
-
Abstract
The growth ability of 80 lactic acid bacteria strains isolated from the traditional fermented food in Inner Mongolia were screened according to the γ- aminobuyric acid( GABA) accumulation amount in the GYP medium.The high- yield GABA strains were obtained by ultraviolet mutagenesis,and then the strain was identified by16 S r DNA gene sequences.The results showed that 4 high- yield GABA strains were screened out from 80 strains of lactic acid bacteria and a high yield GABA producing strain US3- 3 was obtained by UV mutagenesis and its content of GABA was 2.482 g / L,which was 1.9 times as much as original strain.The strain US3-3 was identified as Pediococcus acidilactici.
-
-