Inhibition to advanced glycation end- products and activity evaluation of sap of banana stem

To study the inhibition to advanced glycation end- products( AGEs) activity by sap of banana stem,the inhibition of key enzymes in carbohydrate metabolism was evaluated by using α- glucosidase and α- amylase inhibition model.Inhibitory kinetic parameters were determined by Lineweaver- Burk plot.Meanwhile,the abilities of inhibiting AGEs and removing ABTS⁺· were also evaluated.The results showed that the median inhibitory concentration( IC₅₀) of sap of banana stem on α-glucosidase was 1.51 mg /m L and the type of inhibition was mixcompetitive inhibition. The median inhibitory concentration( IC₅₀) of sap of banana stem on α- amylase was8.64 mg / m L and the type of inhibition was irreversible inhibition. Through experiment,sap of banana stem had a scavenging activity on removing ABTS⁺·also had a certain capacity of inhibiting AGEs.The sap of banana stem had a certain inhibitory activity against AGEs.