Optimization of extraction technology for hyperoside,isoquercitrin,quercetin in Abelmoschus manihot(L.) Medic.by response surface method

The response surface methodology experiments were used to optimize hyperoside,isoquercitrin,and quercetin from Abelmoschus manihot(L.) Medic. Based on single factor experiments,including ultrasonic power,extraction time,ethanol concentration,extraction temperature,solid-liquid rate,response surface method was used to establish polynomial regression equation model with 50 ℃ and 0.1∶15 g/m L. The optimal condition were340 W,extraction time 60 min,and ethanol concentration 60%. Under such extraction technological conditions,the extracting rates of hyperoside,isoquercitrin and quercetin were 4.97 mg/g(RE=2.5%),4.84 mg/g(RE=4.5%)and 2.02 mg/g(RE=6.9%). Used response analysis to obtain the optimum process conditions of extracting surface design could be used to provide theoretical reference for industrialized extraction.