Selection and identification of yeast strains in Daqu liquor in the north of Anhui province

In this experiment,traditional method of isolation and cultivation was adopted to get excellent Daqu yeast strains. 89 dominant yeast strains had been isolated from the spring medium-high-temperature Daqu of some liquor-making enterprise in the north of Anhui province,and then 6 better-growing strains were chosen for secondary screening. Finally 2 representative yeasts strains(N4,N6),which had preponderant growth and stronger alcohol-producing abilities,were picked out through several conventional methods of analysis such as bacterial colony,cellular morphology,features of physiology and biochemistry. After using 18S-ITS r DNA sequencing and phylogenetic analysis, researchers identified N4 as Cryptococcus magnus, and N6 as Sporidiobolus pararoseus. They belonged to the genus of Sporidiobolus. The 2 selected yeasts strains were important strains resources for liquor-production in the north of Anhui province and they were of importance in application.