ESR detection method of radical scavenging activity of blueberry extracts

Objective: The scavenging radical ability of blueberry fruits and leaves extract was compared and analyzed.Method: Electron spin resonance( ESR) spectroscopy was used to measure hydroxyl radical( ·OH) in transient time and 1,1- diphenyl-2- picryllhydrazyl radical( DPPH) in 3~45 min scavenging ability of blueberry fruits and leaves extract at different concentrations. Results: The result showed that scavenging capacity of OH radical( 87%) of blueberry leaves extract was higher than blueberry fruits extract at concentration of 2.500 mg /L and treatment time of 2 min.Scavenging capacity of DPPH radical( > 80%) of blueberry fruits extract was higher than blueberry leaves extract at concentration of 2.500 g / L and treatment time of 30 min.Conclusion: Extract of blueberry had strong scavenging radical ability,which was certainly correlated with the contents of total polyphenol and its composition.