Quantification for 5 selected biogenic amines in fermentation and distillation of Chinese liquor by HPLC

A liquid- liquid microextraction( LLME) and reverse- phase high performance liquid chromatogramphy( HPLC) method was developed for the quantification for 5 selected biogenic amines in fermented grain and Chinese raw liquor. Quantification method generally includes: oscillation extraction,centrifugation,liquid- liquid microextraction,precolumn derivatization with Dansyl chloride,and RP- HPLC detection.The results showed that the content of putrescine and cadaverine was increased with fermentation time prolong of fermented grains,but few of them could be distilled into raw liquor.Pyrrolidine was the major biogenic amines in Chinese liquors and most could be distilled into raw liquor.Methylamine and ethylamine were detemined in fermented grains,but low concentration of them could exist in raw liquor because of their high volatility.Compared with other alcoholic beverages,Chinese liquor has the lowest content of biogenic amines.