Study on extraction of total flavonoids from pomegranate leaf by surfactant

While the pomegranate leaf were used as the research object, the optimum conditions for extraction of total flavonoids from pomegranate leaf were investigated by the single factors and orthogonal array design experiment, and the effect of surfactant kinds, concentration of surfactant and ethanol, extracting temperature, extracting time, liquid- to- material and extracting times on yield of total flavonoids from pomegranate leaf were investigated. The optimum extraction conditions were determined as 2.4g /100 m L, 40%, 1 ∶ 30 ( g / m L) , 60℃ and60 min for tween- 40 concentration, ethanol concentration, solid / liquid ratio ( g / mL) , temperature and extraction time, respectively.Under the optimized conditions, the extraction rate of total flavonoids from pomegranate leaf was86.17% by extracting once.