Genome shuffling and its application in fungal-breeding

The technology of genome shuffling was a novel whole genome engineering approach for the rapid improvement of cellular phenotypes. Based on the recursive protoplast fusion with multi-parental strains, it offers the advantage of recombination throughout the entire genome without the necessity for genome sequence data or network information. This article introduced the principle, characteristics and process of genome shuffling, summarized its application in fungal-breeding and prospected the outlook of it.