Optimization of reverse micelles extraction protein from panda bean by response surface method

The conditions of cetyl trimethyl ammonium bromide (CTAB) reversed micelles extraction protein from panda bean were optimized using Plackett Burman (PB) design, steepest ascent design and central composite design. Firstly, PB design was applied to screen ionic strength, protein concentration and extraction temperature as the three significant ones from seven factors influencing the extraction efficiency of panda bean protein.Then the center points of the three factors were determined based on steepest ascent design. Finally, the optimization of the three factors was carried out by using central composite design. The results showed that the optimal extraction conditions were as follows:extraction time 2h, CTAB concentration 40mmol/L, alkyl ratio 4∶1, pH7.0, protein concentration 1.4mg/mL, extraction temperature 25.7℃, ionic strength 0.02mol/L. Under such conditions, the extraction rate of panda bean protein was 66.7%, which was in good agreement with the predicted one of 64.3%. Backward extraction on the basis of before extraction results using pH4.0, 3mol/L NaCl, aqueous phase temperature 22℃, the backward extraction rate was 53.41%. In conclusion, the optimized process had good reliability.