Study on the speciation of the Zinc in Grifola frondosa

Objective: To study the speciation of the Zinc in Grifolan frondosa.Methods: The working was analyzed by means of the single factor experiment and orthogonal experiment, extraction conditions were optimized and the content and speciation of trace element Zn in its water leaching were analyzed and discussed.By using 0.45μm filter membrane and D- 101 macroreticular resins, four different species of the water leaching, namely the suspended, the soluble, the inorganic and organic species were obtained and separated.In the test, the sample was digested by using microwave digestion system, and the content of the Zinc in those species were determined by FAAS, respectively.Results: The best extracting condition was the ratio of material and liquid was 1 ∶ 45, the temperature was 40℃, the extraction time was 110min, and the extraction rate 78.77% was obtained in this condition.The soluble Zn was about 99.31% in its water leaching, and the inorganic Zn and organic Zn was about78.03% and 22.39%, respectively.Conclusion: The trace element Zn in G.frondosa was existing mainly in form of the inorganic.