Effects of cultural conditions on the growth of Clostridium butyricum

Effects of fermentation conditions such as medium composition, incubation time, inoculation amount and temperature.On the growth of Clostridium butyricum were investigated for improving the growth of Clostridium butyricum and reducing the cost.The conditions of carbon nitrogen ratio and the concentrations of inorganic ions were optimized.The results showed that optimized culture medium was as follows:1.5% corn flour, 1% soybean meal, 0.4% K2HPO4, 0.05% MgSO4, 0.02% MnSO4.The high biomass production of Clostridium butyricum was obtained by heat-treated inoculation at 95℃, pH7.0 and inoculation amount of 6% at 36h incubation.The productivity of viable count and the spore rate of Clostridium butyricum were finally increased by approximately 4.7- fold and 52%, respectively, under the optimal conditions.