Determination of Aflatoxin B1 in vegetable oil by LC-MS/MS

Objective:To establish an LC-MS/MS method to determine the content of Aflatoxin B1 in vegetable oil.Methods:Aflatoxin B1 was extracted from vegetable oil by acetonitrile.The analysis was performed on a ZORBAX SB-C18 column.The mobile phase consisted of acetonitrile-0.2% methane acid solution for gradient elution.The flow rate was 0.3mL/min.Then 5μL was injected for analysis, Electrospray ionization (ESI) sources was applied and operated in the position ion mode.Multiple reaction monitoring (MRM) mode with the transition of m/z 313.0→285.0 and m/z 313.0→240.0 were used to quantify Aflatoxin B1.Results:The sample was in good linear at the range of 0.204⁴.08ng/mL (r=0.9991) .The average recovery rate was 98.15% (n=9) .The RSD was 1.12 %.Conclusion : The method was simple, quick and accurate, proved to be applyable in the content determination of Aflatoxin B1 in vegetable oil.