Optimization of the enzymatic extraction process of protein in tea residue by response surface methodology

To optimize the extraction parameters of protein in tea residue, the Central Composite Design was used to analyze the factors that affect the protein extraction rate.Alcalase was selected to extract the protein in tea residue.Based on single factor experiment results, enzyme hydrolysis temperature, reaction time, the ratio of liquid to solid, enzyme amount and enzyme hydrolysis pH were chosen as influencing factors and the protein yield was selected as the response value.The results showed that the optimal extraction conditions were:enzyme hydrolysis temperature 60℃, reaction time 1.5h, the ratio of liquid to solid 20∶ 1, enzyme amount 2.5% and enzyme hydrolysis pH9.5.In this optimal process condition, the yield of protein in tea residue was 56.95%.Compared with the previous report, extraction rate of protein in tea residue increased significantly with the application of response surface analysis methodology.