Effect of nitrogen, phosphrus and sea salt on the cell growth and lipid accumulation of Micractinium reisseri

In order to study the influence of nutrient contents on the cell growth and lipid accumulation of Micractinium reisseri-a oleaginous microalgae strain from fresh water, effect of nitrogen, phosphrus and sea salt based on BG11 medium for Micractinium reisseri were investigated in the study.The results showed that ammonium chloride was the optimum nitrogen source, with which the lipid productivity of Micractinium reisseri was 1.6 times higher than sodium nitrate which is the original nitrogen source in BG11 medium.Without any nitrogen sources, the lipid concent of Micractinium reisseri was highest, but the biomass was least.When 80mg/L concentrations of phosphrus presented in the culture medium, the lipid productivity of Micractinium reisseri was 1.4 times higher than 40mg/L concentration which was the original concentration in BG11 medium.Sea salt could improve the growth of Micractinium reisseri, and Micractinium reisseri could adapt to high salinity (20g/L) well.The optimum concentration of sea salt was 10g/L, in which concentration the lipid productivity was highest in all and 2.4 times higher than without any addition of sea salt.