Development of an enzyme linked immunosorbent assay kit for furantoin metabolite residues determination in animal tissue

Artificial hapten and immunogen were synthesized, after which monoclonal antibodies against furantoin metabolite was raised.On the basis of furantoin metabolite monoclonal antibody, rapid test enzyme linked immunosorbent assay (ELISA) kit for furantoin metabolite residues in animal tissue was developed.The sensitivity and detection limit of ELISA kit were 0.05μg/L and 0.1μg/kg, respectively.Sample recoveries were 78.2%～102.7%, and the coefficients of variation were 5.1%～11.4%.The cross-reactivity towards furantoin was 13%.As for other drugs, the cross-reactivity was below 1%.The results of ELISA kit and liquid chromatographic tandem mass spectrometry (LC-MS/MS) were in accordance.This method was rapid, sensitive and reliable, which provided a convenient and accurate way for detection of furantoin metabolite residues.