Optimization of culture medium to produce manganese peroxidase by using Box-Behnken experimental design and response surface methodology

White rot fungi preserved in our laboratory was used to produce manganese peroxidase.Design Expert 8.05 software was used to optimize the culture medium producing this peroxidase.3-Level factorial analysis was used to analyze the main effect factors from 10 factors.The result showed that the wheat bran, yeast extract and KH 2 PO 4 were the significant factors.According to Box-Behnken experimental design and response surface methodology, the second-order equation model was established by regression analysis of experimental data.The predicted optimal addition of wheat bran, yeast extract and KH 2 PO 4 were 10.75、3.37 and 0.095g/L respectively, and the predicted maximum manganese peroxidase could reach 4.06U/mL.The manganese peroxidase activity of verification experiment was 4.15U/mL, which was very close to the predicted value.The manganese peroxidase activity under the optimization condition increased by 58.4% compared with the original enzyme production medium.