Optimization of emzyme-assisted extraction processing of oil from pumpkin seed by response surface methodology

The cellulase-assisted extraction processing of oil from pumpkin seed was studied.On the basis of single -factor, the extraction conditions of oil from pumpkin seed by cellulase was optimized through Box-Benhnken design and response surface methodology.The mathematical regress model was established about extraction rate and enzyme dosage, time and temperature. According to this model, the optimum extraction conditions were determined as enzyme dosage 17mg /g, time 2.64h and temperature 47℃ .The extraction rate for this optimum condition was 89.12%.