Method optimization of content determination of the main components in natural apple essence

ObjectiveEstablished the method to determine the contents of 14 main compounds in natural apple essence using Head Space-Solid Phase Microextraction, and gas chromatography mass spectrometry.Method The optimal HS-SPME condition was obtained through single factor and surface response design experiments.Standard curve was established by internal standard method to determine compounds'contents.ResultThe optimal conditions for HS-SPME were 85μm PA fiber, 2mL natural apple essence, 40℃ water bath, 0.4g/mL NaCl, 30min of extracting time.Results of content determination of compounds experiments:the precision experiment results RSD was 1.20%～3.60%;the repeatability experiment results RSD was 3.17%～8.86%;the recovery of chemical standards was 93.44%～107.42%.ConclusionThe method of HS-SPME/GC-MS coupled with internal standard curve provides high accuracy, precision, repeatability, was effective for content determination for compounds in apple essence.