PCR-DGGE analysis on the dynamic changes of microorganisms in cutlassfish (Trichiurus haumela) under the cold storage

In this paper, the main microbe dynamic changes of cutlassfish (Trichiurus haumela) were studied under the cold storage (4±1) ℃so as to provide the experimental references for extending its shelf-life.The total bacterium DNA of Trichiurus haumela were extracted by SDS (Sodium Dodecyl Sulfate) pyrolysis method and the PCR amplification of V3 variable region of 16S rRNA were taken in order to get the dynamic changing fingerprint spectra by DGGE technology.It was claimed that there were abundant microbial population on the products in the initial stage and Psychrobacter sp.was the main bacterium firstly.The proportion of Pseudomonas fluorescens was increased from the initial periods.With the extension of storage time, the proportion of Shewanella sp.and Vibrio sp.also increased gradually and they took the place of Psychrobacter sp.to be the dominant bacteria finally.