丝胶肽F5-SPs对<i>α</i>-葡萄糖苷酶的抑制作用及其稳定性

杨迎; 金泉; 罗静; 黄心阳; 许蓓; 龚盛祥; 张维; 房郁雯; 王正武; 吴金鸿; 史海明

丝胶肽F5-SPs对α-葡萄糖苷酶的抑制作用及其稳定性

The stability and inhibitory effect of F5-SPs derived from sericin peptides on α-Glucosidase

摘要

摘要: 以丝胶肽中的F5-SPs对α-葡萄糖苷酶的抑制活性为考察指标,考察了反应温度、pH、紫外光照时间和人工消化液对丝胶肽F5-SPs稳定性的影响。结果表明F5-SPs在较高温度下(>37 ℃)稳定性良好;在强酸强碱的条件下,F5-SPs对α-葡萄糖苷酶活性的抑制率明显降低;紫外光照对F5-SPs影响较小。此外,F5-SPs在经过两种人工消化液的处理后,对α-葡萄糖苷酶的抑制活性都有一定程度的损失。本文研究显示新发现的具有α-葡萄糖苷酶抑制活性的丝胶肽F5-SPs对温度、pH、紫外光照以及人工消化液都有一定的耐受性,具有很大的应用开发潜力。

Abstract: Tooking the inhibitory activity of F5-SPs derived from serine peptides on α-glucosidaseas the investigated index,the effects of temperature,pH,time of ultraviolet and artificial digestion on the stability of F5-SPs were investigated. The results showed that F5-SPs were stable at higher temperatures(>37 ℃). In the case of strong acid and strong alkali,the inhibitory activity of F5-SPs on α-glucosidase was significantly reduced. The effect of UV irradiation on F5-SPs was moderate. In addition,the F5-SPs had a certain degree of loss of α-glucosidase inhibitory activity after treatment with two kinds of artificial digestive solution. Sericin peptide F5-SPs has a certain toleranceon the temperature,pH,ultraviolet and artificial digestive solution. The result showed that the newly discovered F5-SPs derived from serine peptides with α-glucosidase inhibitory activity had a certain tolerance to temperature,pH,ultraviolet and artificial digestive solution,and had great potential for application and development.