白灵菇多糖对果蝇寿命及抗氧化活性的影响

王耀辉; 任海虹; 王景雪; 袁凌; 邱现创; 段梦妮

白灵菇多糖对果蝇寿命及抗氧化活性的影响

Effect of polysaccharides of Pleurotus nebrodensis on the life-span and antioxidant activities of Drosophila melanogaster

摘要

摘要: 目的:研究白灵菇多糖对果蝇寿命的影响。方法:本实验使用黑腹果蝇作为动物模型,通过研究白灵菇多糖对黑腹果蝇体重、逆重力爬行能力、寿命和体内酶活力的影响,评价白灵菇多糖的抗衰老作用。结果:与对照组相比,基础培养基中添加0.50%白灵菇多糖能显著增加果蝇的逆重力爬行能力(p<0.01)和果蝇的体重(p<0.05);显著提高果蝇的平均寿命(雌果蝇增加22.77%,雄果蝇增加21.01%,p<0.05)、半数死亡时间(雌果蝇增加23.08%,雄果蝇增加27.86%,p<0.01)、最高寿命(雌果蝇增加18.24%,雄果蝇增加20.05%,p<0.01)。果蝇体内抗氧化性测定结果显示,与对照组相比,0.50%白灵菇多糖剂量组喂养30 d的雌性和雄性果蝇,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性均升高,丙二醛(MDA)含量下降均达到显著水平(p<0.05)。结论:白灵菇多糖可通过提高机体抗氧化酶活性,减少脂质过氧化作用,延缓衰老,延长寿命。

Abstract: Objective:To study the effect of Pleurotus nebrodensis polysaccharide on the life-span of Drosophila melanogaster. Methods:In this study,the effects of polysaccharides from P. nebrodensis on average body weight,reverse gravity crawling ability,longevity and anti-oxidation activity of Drosophila melanogaster were studied.Results:Compare with the control,feeding with 0.50% of P. nebrodensis polysaccharide significantly increased the reverse gravity crawling ability(p<0.01)and the average body(p<0.05)weight of D. melanogaster. The average life-span of D. melanogaster(females increased by 22.77%,males increased by 21.01%,p<0.01),the half-death phase(females increased by 23.08% and males increased by 27.86%,p<0.01),and the longest life-span(females increased by 18.24% and males increased by 20.05%,p<0.01). The test of antioxidant activities of D. melanogaster which was fed in 30 days showed that the activities of superoxide dismutase(SOD)and catalase(CAT)were increased significantly and the MDA content decreased significantly in 0.50% dose group(p<0.05). Conclusion:The PNPs could increase a series of antioxidant enzyme activity,reduce lipid peroxidation,to extend D. melanogaster longevity pathways.