浒苔多糖铁的制备工艺优化及其结构表征

Optimization of preparation process of Enteromorpha prolifera polysaccharide-iron and its structure characterization

摘要: 通过响应面法优化浒苔多糖铁的螯合工艺,并利用红外光谱法和X衍射分析浒苔多糖铁的结构。结果表明,浒苔多糖铁的最佳制备工艺为反应温度54 ℃,螯合时间3.8 h,浒苔多糖与三价铁比例3.9∶1,pH7.5,在此条件下螯合率为19.6%。红外光谱表明浒苔多糖中的O-H和C=O与Fe³⁺发生了螯合。X射线衍射结果进一步证实了浒苔多糖铁的生成。从而为拓展浒苔资源的高值利用及开发新型多糖补铁剂提供了一定的科学依据。

Abstract: The optimal chelating conditions for Enteromorpha prolifera polysaccharide-iron were investigated by response surface methodology(RSM). It was characterized through fourier transform infrared(FTIR)spectroscopy and X-ray diffraction. Results showed that the optimal reaction conditions were temperature 54 ℃,chelating time 3.8 h,the mass ration of polysaccharide and ferric iron 3.9∶1,and pH7.5. The chelating rate could reach 19.6%. The FTIR indicated that the hydroxyl and carboxyl groups were chelated with Fe³⁺. The generation of E. prolifera polysaccharide-iron was further confirmed by X-ray diffraction to provide scientific basis for the high-value utilization of E. prolifera resource and the exploitation of a new iron supplement.