溪蜜柚精油的抗氧化及其对B16细胞中酪氨酸酶的抑制效果

李小燕; 何婉莺; 彭颖; 刘畅; 潘思轶

溪蜜柚精油的抗氧化及其对B16细胞中酪氨酸酶的抑制效果

Antioxidant and tyrosinase inhibitory activity in B16 cells of Guanxi pomelo essential oil

摘要

摘要: 本研究采用冷压法制备琯溪蜜柚精油,并对比分析了蜜柚精油的抗氧化性及精油抑制酪氨酸酶的效果。结果表明,在150 mg/mL时,精油对DPPH自由基清除率达到了68%,低于同浓度的BHT的清除率,半抑制浓度为70.12 mg/mL;在3 mg/mL时,精油对羟自由基清除率达到了92%,高于同浓度的V_C的清除率,半抑制浓度为0.88 mg/mL;当质量浓度为1.0 mg/mL时,精油总抗氧化性能比BHT高出20.35%。而当精油质量浓度大于100 μg/mL时,酪氨酸酶激活率小于曲酸,半抑制浓度为157.45 μg/mL,具有较好的抑制B16细胞酪氨酸酶作用。由于蜜柚精油有良好的抗氧化性与美白的作用,因此在食品与化妆品等中有较好的应用前景。

Abstract: In this study,the oil of Guanxi pomelo oil(GP oil)was prepared by cold pressing method,and the antioxidant activity and the effect on tyrosinase of pomelo oil were compared and analyzed. The results showed that the DPPH free radical scavenging rate of GP oil reached 68% at 150 mg/mL which was lower than that of BHT,IC₅₀ was 70.12 mg/mL. At 3 mg/mL,the hydroxyl free radical scavenging rate of GP oil was 92%,which was higher than that of V_C at the same concentration,IC₅₀ was 0.88 mg/mL;when the mass concentration was 1.0 mg/mL,the total antioxidant activity of GP oil was 20.35% higher than that of BHT. And the activation rate of tyrosinase was lower than that of kojic acid when the concentration of GP oil was more than 100 μg/mL,IC₅₀ was 157.45 μg/mL. It means that the GP oil could inhibit the tyrosinase effect of B16 cells. As the GP oil has a good antioxidant and whitening effect,it had a better application prospects in food and cosmetics.