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中国精品科技期刊2020
阳景阳,陈远权,梁贤智,等. HS-SPME-GC-MS结合OAV对乌龙茶树品种制花香型红茶的香气评价[J]. 华体会体育,2025,46(6):1−10. doi: 10.13386/j.issn1002-0306.2024050077.
引用本文: 阳景阳,陈远权,梁贤智,等. HS-SPME-GC-MS结合OAV对乌龙茶树品种制花香型红茶的香气评价[J]. 华体会体育,2025,46(6):1−10. doi: 10.13386/j.issn1002-0306.2024050077.
YANG Jingyang, CHEN Yuanquan, LIANG Xianzhi, et al. HS-SPME-GC-MS Coupled with OAV to Evaluate the Aroma of Flowery Black Tea Made from Oolong Tea Varieties[J]. Science and Technology of Food Industry, 2025, 46(6): 1−10. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024050077.
Citation: YANG Jingyang, CHEN Yuanquan, LIANG Xianzhi, et al. HS-SPME-GC-MS Coupled with OAV to Evaluate the Aroma of Flowery Black Tea Made from Oolong Tea Varieties[J]. Science and Technology of Food Industry, 2025, 46(6): 1−10. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024050077.

HS-SPME-GC-MS结合OAV对乌龙茶树品种制花香型红茶的香气评价

HS-SPME-GC-MS Coupled with OAV to Evaluate the Aroma of Flowery Black Tea Made from Oolong Tea Varieties

  • 摘要: 为探究乌龙茶树品种制作花香型红茶的挥发性成分组成和呈香物质差异。本研究采用顶空固相微萃取-气质联用技术(headspace-solid phase microextraction-gas chromatography-mass spectrometry,HS-SPME-GC-MS)鉴定茶叶香气,气味活度值(odor activity value,OAV)法确定关键及修饰性风味组分,结合层次聚类分析(hierarchical clustering analysis,HCA)、主成分分析(principal component analysis,PCA)、正交偏最小二乘判别分析(orthogonal partial least squares discrimination analysis,OPLS-DA)及感官审评对花香型红茶香气进行对比评价。结果表明,从鉴定出的54种香气成分中筛选出22种关键及修饰性风味组分,层次聚类将其分为五类;PCA模型(R2X(cum)=0.892,Q2(cum)=0.645)和OPLS-DA模型(R2X(cum)>0.7,Q2(cum)>0.9)解释率较高,传统品种红茶与乌龙茶品种所制花香型红茶差异香气成分为芳樟醇、芳樟醇氧化物I、芳樟醇氧化物II和2-甲基丁醛,花香型红茶与同品种乌龙茶的差异成分为β-环柠檬醛、芳樟醇氧化物I、(E,E)-2,4-壬二烯醛、己醛、1-戊烯-3-酮、芳樟醇氧化物II、芳樟醇和香叶醇;OAV法结合化学计量学方法对目标茶类香气特点的分析判断与感官评价基本契合。本试验结果可为明确花香型红茶呈香物质差异以及优化挥发性成分分析方法提供参考。

     

    Abstract: To analyze the difference in aroma components and aroma characteristics of flowery black tea made from oolong tea varieties. The aroma components extracted with headspace-solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS), and odor activity value (OAV) was used to determine the key and modified flavor components. Combined with hierarchical clustering analysis (HCA), principal component analysis(PCA), orthogonal partial least squares discrimination analysis (OPLS-DA) and sensory evaluation, the aroma of flowery black tea was comprehensively evaluated. Results showed that 22 key and modified flavor components were selected from 54 identified aroma components, hierarchical clustering divided them into five categories; The models of PCA (R2X(cum)=0.892, Q2(cum)=0.645) and OPLS-DA (R2X(cum)>0.7, Q2(cum)>0.9) had a high degree of fitting. The main difference components between traditional black tea and flowery black tea were linalool, linalool oxide I, linalool oxide II and 2-methylbutyraldehyde. The main difference components between flowery black tea and oolong tea were β-cyclocitral, linalool oxide I, trans,trans-nona-2,4-dienal, hexanal, 1-penten-3-one, linalool oxide II, linalool and geraniol. Determination of flowery black tea aroma characteristics by OAV combined with chemometrics methods was consistent with the sensory results. The results of this experiment can clarify the differences in volatile substances in flowery black tea and provide for further optimization of volatile component analysis methods.

     

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